Sperm motility and dilution effect

The aim of this study was to evaluate the effects of dilution rate and holding time on various motility parameters using computer-assisted sperm analysis CASA. The semen samples were collected from three Ghezel rams. In all diluters, there was a decrease in the average of all three parameters of sperms movement velocity as the time passed, but density of this decrease was more intensive in SP. The average of LIN in the diluted sperms in Bioexcell was less than two other diluters in all three times. The motility parameters of the diluted sperms in Bioexcell and PBS indicated an important and considerable difference with the diluted sperms in SP. According to the gained results, the Bioexcell has greater ability in preserving motility of sperm in comparison with the other diluters but as SP is considered as physiological environment for sperm.
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Bovine serum albumin, sperm motility, and the "dilution effect'.

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Effects of diluting medium and holding time on sperm motility analysis by CASA in ram

Skip to search form Skip to main content. Bovine serum albumin, sperm motility, and the "dilution effect'. View on PubMed. Alternate Sources. Save to Library. Create Alert.
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Effects of diluting medium and holding time on sperm motility analysis by CASA in ram

The sperm quality index SQI is a tool used to predict overall rooster semen quality, fertility, and hatchability. However, semen must be diluted before SQI analysis, and research has shown that the SQI is most predictive of fertility at lower semen dilutions. Therefore, the present study was undertaken to determine why the SQI is not as predictive of fertility at higher semen dilutions and whether semen diluent type alters the SQI, adenosine triphosphate ATP utilization, gas exchange, and ionic balance of broiler breeder sperm.
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Epididymal spermatozoa from rabbit and ram were washed either once or twice using an efficient washing procedure and were then diluted in various media to a final concentration of approximately 1. Bovine serum albumin BSA , either untreated or defatted, was found to be better than polyvinylpyrrolidone, ovalbumin, or alpha-lactalbumin, both at stimulating and maintaining motility levels and at reducing the tendency of the washed spermatozoa to stick to glass. BSA has a reversible stimulatory effect on motility. However, samples washed into BSA and then washed free of it behaved essentially as though they had never been in contact with protein.
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Comments
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